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Image Search Results
Journal: Frontiers in Immunology
Article Title: Production of IL-6 and Phagocytosis Are the Most Resilient Immune Functions in Metabolically Compromised Human Monocytes
doi: 10.3389/fimmu.2021.730672
Figure Lengend Snippet: Antibodies used for flow cytometry.
Article Snippet: anti-hIL-6 ,
Techniques: Cytometry, Recombinant
Journal: Journal of Cellular and Molecular Medicine
Article Title: Complement 5a‐mediated trophoblasts dysfunction is involved in the development of pre‐eclampsia
doi: 10.1111/jcmm.13466
Figure Lengend Snippet: Expression of angiogenesis‐related factors in human placenta. ( A ) Relative mRNA levels of anti‐angiogenic factors ( IL ‐1β, TNF ‐α, IL ‐6, MCP ‐1 and sF lt1) and pro‐angiogenic ( PIGF , IL ‐10) in normal and pre‐eclamptic placentas. Data are represented as mean ± S.E.M. N = 4–6 in each group. * P < 0.05, ** P < 0.01, *** P < 0.001. ( B ) Immunofluorescence analysis of representative angiogenesis‐related factors (red) and Cy7 (green) in normal and PE placentas. Nuclei were counterstained with DAPI (blue). Scale bar: 60 μm.
Article Snippet: The human placenta slices were blocked in 10% normal goat serum for 30 min., then incubated with primary antibodies against CD31 (Santa Cruz), CD11b (BD Biosciences, San Jose, CA, USA), C5a (Comp Tech, A221), C5aR (Biolegend), cytokeratin 7 (Cy7, Santa Cruz), PIGF (Proteintech group, Wuhan, Hubei, China), sFlt1 (Life Technologies, Waltham, MA, USA), IL‐1β (Boster),
Techniques: Expressing, Immunofluorescence
Journal: Journal of Cellular and Molecular Medicine
Article Title: Complement 5a‐mediated trophoblasts dysfunction is involved in the development of pre‐eclampsia
doi: 10.1111/jcmm.13466
Figure Lengend Snippet: Trophoblasts stimulated with C5a display an anti‐angiogenic phenotype. ( A ) HTR ‐8/ SV neo cells treated with C5a showed a polarization towards an anti‐angiogenic phenotype with significantly increased mRNA levels of IL ‐1β, TNF ‐α, IL ‐6, MCP ‐1, sF lt1 and decreased mRNA level of PIGF and IL ‐10. The respective mRNA was normalized to β‐actin housekeeping gene. Data are represented as mean ± S.E.M . N = 3 in each group * P < 0.05, ** P < 0.01. ( B ) Immunofluorescence staining for sF lt1 and PIGF expression in HTR ‐8/ SV neo cells in the presence of C5a or PBS ( CON ). Scale bar: 60 μm.
Article Snippet: The human placenta slices were blocked in 10% normal goat serum for 30 min., then incubated with primary antibodies against CD31 (Santa Cruz), CD11b (BD Biosciences, San Jose, CA, USA), C5a (Comp Tech, A221), C5aR (Biolegend), cytokeratin 7 (Cy7, Santa Cruz), PIGF (Proteintech group, Wuhan, Hubei, China), sFlt1 (Life Technologies, Waltham, MA, USA), IL‐1β (Boster),
Techniques: Immunofluorescence, Staining, Expressing